PAK1KD (recombinant human PAK1 kinase domain)

Catalog #:	Size:	Concentration:	Price:
K0104S	10 µg	1 mg/ml	$330
K0104M	100 µg	1 mg/ml	$2,500
K0104L	1 mg	1 mg/ml	$8,000
K0104B	Bulk	1 mg/ml	Inquiry

To Order:

Please email PO to order@accelagen.com or fax PO to 858-678-8628

PRODUCT DETAIL

DATA SHEET

Sequence:
GPHPSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMREN
KNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGM
DGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIA
TNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFLKIAKPLSSLTPLIAAAKEATKNNH

The first 4 residues GPHP are from Turbo3C cleavage site.
Phosphorylated at T423.

Component and Formulation:

PAK1KD: 1 mg/ml in Storage Buffer of 25 mM Tris-HCl pH8.0, 150 mM NaCl, 10% glycerol, 5 mM DTT.

Activity:

Specific activity:
32,638 pmoles/min/µg

Analysis of enzymatic activity was performed according to Zlyte assay protocol (Invitrogen):

Different concentration of PAK1KD were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl₂, 1 mM EGTA, 200 mM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 14, Invitrogen) at room temperature for 1 hour.
Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT.
Fluorescence was detected using lexc=460±40 nm and lem=528±20 nm filters.

SDS-PAGE Analysis:

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