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border="0" cellspacing="0" cellpadding="0"> <tr height="42" bgcolor="#B3D9FE"> <td><b>Catalog #:</b></td> <td><b>Size:</b></td> <td><b>Concentration:</b></td> <td><b>Price:</b></td> <td> <!--View Cart Button--> <form target="paypal" action="https://www.paypal.com/cgi-bin/webscr" method="post"> <input type="hidden" name="cmd" value="_s-xclick"> <input type="hidden" name="encrypted" value="-----BEGIN PKCS7-----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-----END PKCS7----- "> <input type="image" src="https://www.paypalobjects.com/en_US/i/btn/btn_viewcart_LG.gif" border="0" name="submit" alt="PayPal - The safer, easier way to pay online!"> <img alt="" border="0" src="https://www.paypalobjects.com/en_US/i/scr/pixel.gif" width="1" height="1"> </form> <!--View Cart Button end--> </td> </tr> <tr> <td valign="top">K0105S</td> <td valign="top">10 µg</td> <td valign="top">1 mg/ml</td> <td valign="top">$330</td> <td> <!-- Paypal button --> <form target="paypal" action="https://www.paypal.com/cgi-bin/webscr" method="post"> <input type="hidden" name="cmd" value="_s-xclick"> <input type="hidden" name="hosted_button_id" value="4UQ3C2Z5UJY6J"> <input type="image" src="https://www.paypalobjects.com/en_US/i/btn/btn_cart_LG.gif" border="0" name="submit" alt="PayPal - The safer, easier way to pay online!"> <img alt="" border="0" src="https://www.paypalobjects.com/en_US/i/scr/pixel.gif" width="1" height="1"> </form> </td> </tr> <tr> <td valign="top">K0105M</td> <td valign="top">100 µg</td> <td valign="top">1 mg/ml</td> <td valign="top">$2,500</td> <td> <!-- Paypal button --> <form target="paypal" action="https://www.paypal.com/cgi-bin/webscr" method="post"> <input type="hidden" name="cmd" value="_s-xclick"> <input type="hidden" name="hosted_button_id" value="UPR79YJ58AKQL"> <input type="image" src="https://www.paypalobjects.com/en_US/i/btn/btn_cart_LG.gif" border="0" name="submit" alt="PayPal - The safer, easier way to pay online!"> <img alt="" border="0" src="https://www.paypalobjects.com/en_US/i/scr/pixel.gif" width="1" height="1"> </form> </td> </tr> <tr> <td valign="top">K0105L</td> <td valign="top">1 mg</td> <td valign="top">1 mg/ml</td> <td valign="top">$8,000</td> <td> <!-- Paypal button --> <form target="paypal" action="https://www.paypal.com/cgi-bin/webscr" method="post"> <input type="hidden" name="cmd" value="_s-xclick"> <input type="hidden" name="hosted_button_id" value="29PP99R45BHE2"> <input type="image" src="https://www.paypalobjects.com/en_US/i/btn/btn_cart_LG.gif" border="0" name="submit" alt="PayPal - The safer, easier way to pay online!"> <img alt="" border="0" src="https://www.paypalobjects.com/en_US/i/scr/pixel.gif" width="1" height="1"> </form> </td> </tr> <tr> <td valign="top">K0105B</td> <td valign="top">Bulk</td> <td valign="top">1 mg/ml</td> <td valign="top"><a href="mailto:order@accelagen.com">Inquiry</a></td> </tr> </table> <br><hr> <table border="0" cellspacing="0" cellpadding="0"> <tr> <td width="154"><b>To Order: </b></td> <td>Please email PO to <a href="mailto:order@accelagen.com">order@accelagen.com</a> or fax PO to 858-678-8628</td> </tr> </table> <br><br> <table width="300" border="0" cellspacing="0" cellpadding="4"> <tr align="center"> <td width="150" bgcolor="#B3D9FE" class="prod-tab-border" ><strong><a href="PAK2KD.htm">PRODUCT DETAIL </a> </strong></td> <td width="150" bgcolor="#3C63B2"><strong><a href="#"><font color="#ffffff">DATA SHEET</font></a></strong></td> </tr> </table> <table width="100%" border="0" cellpadding="10" cellspacing="0" bordercolor="#2B55AB" class="product-border"> <tr> <td valign="top"> <span class="h2">Sequence:</span><br><br> <span class="coypright"><font color="blue"><u>GPHM</u></font>TDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVFTATDVALGQEVAIKQINLQKQPKKELIINEILVMKE<br> LKNPNIVNFLDSYLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLL<br> GMEGSVKLTDFGFCAQITPEQSKRS<font color="red"><b><u>T</u></b></font>MVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRA<br> LYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGSAKELLQHPFLKLAKPLSSLTPLIMAAKEAMKSNR</span> <ul> <li>The first 4 residues <font color="blue"><u>GPHM</u></font> are from Turbo3C cleavage site.</li> <li>Phosphorylated at <font color="red"><b><u>T</u></b></font>402.</li> </ul> <span class="h2">Component and Formulation:</span> <p>PAK2KD: 1 mg/ml in Storage Buffer of 25 mM Tris-HCl pH8.0, 150 mM NaCl, 10% glycerol, 5 mM DTT.</p> <span class="h2">Activity:</span><br> <TABLE BORDER="0"> <br> <tr> <TD WIDTH="30%"><img src="images/Pak2KD_activity.jpg" width="240" height="160"></TD> <TD WIDTH="70%" align="left" valign="top"> Specific activity:<br> 85,806 pmoles/min/µg <p> Analysis of enzymatic activity was performed according to Zlyte assay protocol (Invitrogen): <ol> <li>Different concentration of PAK2KD were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl<sub>2</sub>, 1 mM EGTA, 200 <font face = "symbol">m</font>M ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 14, Invitrogen) at room temperature for 1 hour. <li>Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT. <li>Fluorescence was detected using <font face = "symbol">l</font>exc=460±40 nm and <font face = "symbol">l</font>em=528±20 nm filters. </ol> </TD> </TR> </TABLE> <span class="h2">SDS-PAGE Analysis:</span><br> <TABLE BORDER="0"> <br> <tr> <TD WIDTH="30%"><img src="images/Pak2KD_gel.jpg" width="250" height="281"></TD> <TD WIDTH="70%" align="left" valign="top"> </TD> </tr> </TABLE> </tr> </table> <p><br> </p> <!-- InstanceEndEditable --></td> </tr> </table> <table width="100%" border="0" cellspacing="0" cellpadding="0"> <tr> <td align="center"><br> <a href="protein-purification.htm" class="footer">Protein Purification</a> | <a href="baculovirus-expression.htm" class="footer">Baculovirus Expression</a> | <a href="mammalian-expression.htm" class="footer">Mammalian Expression</a> | <a href="ecoli-expression.htm" class="footer">E. coli Expression</a> | <a href="molecular-biology.htm" class="footer">Molecular Biology</a> | <a href="proteins.htm" class="footer">Proteins</a><br> <p class="style1"><span class="coypright">tel: 858-678-8618 &nbsp;&nbsp;&nbsp; fax: 858-678-8628 &nbsp;&nbsp;&nbsp;toll-free: 888-365-8085 &nbsp;&nbsp;&nbsp; email:</span> <a href="mailto:info@accelagen.com" class="footer">info@accelagen.com<br> </a><span class="coypright">Copyright © 2012 Accelagen Inc. 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