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Protein Purification
Baculovirus Expression
Mammalian Expression
E. coli Expression
Molecular Biology
Products
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Products: Eznyme Toolbox: Turbo3C
Turbo3CTM Protease (HRV 3C Protease)
| Catalog #: | Size: | Concentration: | Price: |
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| H0101S | 1 mg (1,000 Units) | 2 mg/ml | $360 |
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| H0101M | 10 mg (10,000 Units) | 2 mg/ml | $2,980 |
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| H0101B | Bulk | 2 mg/ml | Inquiry |
Formulation:
2 mg/ml in 25 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM TCEP, and 50% glycerol
Protease Activity:
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A 68 kDa GST-fusion protein (C) at 1 mg/ml is incubated with Turbo3C (HRV3C) Protease (*) at a ratio of (1) 1:50, (2) 1:100, (3) 1:200, (4) 1:400 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4oC for 16 hours. The cleaved products are 42 kDa and 26 kDa
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