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Protein Purification
Baculovirus Expression
Mammalian Expression
E. coli Expression
Molecular Biology
Products
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Products: Eznyme Toolbox: TurboLigation Kit
TurboLigationTM Kit
| Catalog #: | Size: | Price: |
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| L0110S | 100 reactions | $177 |
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| L0110M | 500 reactions | $666 |
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| L0110B | Bulk | Inquiry |
Description:
TurboLigationTM Kit enables quick ligation of both blunt-end or cohesive-end DNA fragments in just 5 minutes at room temperature. The kit combines TurboLigaseTM, an ultra-pure recombinant T4 DNA Ligase, and 5X TurboLigation Buffer, an optimized quick ligation buffer. A 5 minute TurboLigation reaction offers the same efficiency as a traditional 16oC overnight ligation reaction. The reaction product is ready for bacterial transformation without any further treatment.
Applications:
- Adding linkers or adapters to DNA
- Blunt or cohesive end ligation of duplex DNA
- DNA cloning
Components:
TurboLigaseTM: 5 unit/µl in Storage Buffer of 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, and 50% glycerol
5X TurboLigation Buffer: 250 mM Tris-HCl, pH 7.5, 50 mM MgCl2, 5 mM dithiothreitol, 5 mM ATP, 25% polyethylene glycol 8000
Stability and Storage:
TurboLigase and 5X TurboLigation Buffer are stable at -20oC for 12 months from date of receipt.
Store TurboLigase and 5X TurboLigation Buffer at -20oC.
Shipped in gel packs.
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