Protein Purification
Baculovirus Expression
Mammalian Expression
E. coli Expression
Molecular Biology
Products
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Products: Eznyme Toolbox: TurboNuclease
TurboNuclease (Benzonase equivalent, ultra-pure)
recombinant Serratia macescens extracellular endonuclease
(encoded by the same gene of Benzonase)
| Catalog #: | Size: | Concentration: | Price: |
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| N0103M | 50,000 Units | 250 Units/µl | $200 |
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| N0103B | Bulk | 250 Units/µl | Inquiry |
Description:
TurboNuclease is a recombinant form of Serratia macescens extracellular endonuclease (encoded by the same gene of Bezonase) produced in E. coli using a proprietary process. This nonspecific endonuclease hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5’-phosphorylated oligonucleotides of 1-4 bases in length. TurboNuclease is a highly purified homodimer of 27 kDa subunits that has exceptional high specific activity and is free of protease activity. TurboNuclease is ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.
Protein Type:
Endonuclease
Source:
E. coli
Unit Definition:
One unit of TurboNuclease converts 1.0 OD260 of salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37oC in a reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2. This corresponds to complete digestion of 50 µg of salmon sperm DNA into oligonucleotides. TurboNuclease has similar specific activity as Benzonase
Storage:
Store TurboNuclease at -20oC.
Endotoxin Level:
< 0.1 EU / 1,000 units of TurboNuclease as determined by the Endo-Safe LAL Assay. |
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